ABSTRACT
OBJECTIVE@#To explore the relationship between HLA-A, -B, -C, -DRB1, -DQB1 gene polymorphism and aplastic anemia （AA）of 65 cases in Northern China.@*METHODS@#The high resolution genotyping of HLA-A, -B, -C, -DRB1, -DQB1 alleles in 65 AA patients and 772 healthy controls was performed with polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO), the relationship between HLA-A, -B, -C, -DRB1, -DQB1 gene polymorphism and aplastic anemia was analyzed by Pearson Chi-square，Continuity Correction， Two-sided Fisher's Exact Test and Odds Ratio.@*RESULTS@#The HLA-B*1302（10% vs 4.21%）, B*3501（7.69% vs 3.89%）, DRB1* 0701（10% vs 4.73%）, DRB1*0901（19.23% vs 7.58%）, DQB1*0202（9.23% vs 3.76%） gene frequency in AA patients was higher than those in health controls, the difference was statistically significant (P<0.05), the χ were 9.049, 4.336, 6.838, 20.974 and 8.968, OR ratio was 2.528, 2.061, 2.239, 2.904 and 2.605. However, the HLA-A*3303（1.54% vs 6.93%）, DQB1*0302（1.54% vs 6.02%） gene frequency in AA patients was lower than those in healthy controls, the difference was statistically significant (P<0.05), the χ was 5.726 and 4.505, the OR ratio were 0.210 and 0.244.@*CONCLUSION@#The polymorphism of HLA-A, -B, -DRB1, -DQB1 alleles is associate with AA in these patient cases, the HLA-B*1302, HLA-B*3501, HLA-DRB1*0701, HLA-DRB1*0901 and HLA-DQB1*0202 may be sensitive genes to AA, while the HLA-A*3303 and HLA-DQB1*0302 may be protective genes on AA.
Subject(s)
Humans , Alleles , Anemia, Aplastic , Genetics , China , Gene Frequency , Genetic Predisposition to Disease , HLA Antigens , Genetics , Polymorphism, GeneticABSTRACT
@#Objective To explore the possible mechanism of quercetin on apoptosis of hippocampal neurons cultured in high glucose. Methods Hippocampus was obtained from newborn 24 hours Sprague-Dawley rats and then primarily cultured.Then hippocampal neurons were divided into normal control group,high glucose group,quercetin group,quercetin+Akt inhibitor group and Akt agonist group.After each group was cultured in different conditioned medium for 72 hours,they were detected the apoptosis of neurons with flow cytometry,and the expression of Akt,p-Akt,Bcl-2 and Bax with Western blotting.Results Compared with the normal control group,the apoptosis rate of hippocampal neurons increased significantly(P<0.01),and p-Akt/Akt and Bcl-2/Bax decreased significantly(P<0.01)in the high glucose group.Compared with the high glucose group,the apoptosis rates of hippocampal neurons decreased significantly(P<0.01),and p-Akt/Akt and Bcl-2/Bax increased significantly(P<0.01)in the quercetin group and the Akt agonist group.Compared with the quercetin+Akt inhibi-tor group,the apoptosis rate of hippocampal neurons decreased significantly(P<0.01),and p-Akt/Akt and Bcl-2/Bax increased significantly (P<0.01)in the quercetin group.Conclusion Quercetin could reduce the apoptosis of hippocampal neurons cultured in high glucose,which may be achieved by increasing the phosphorylation of Akt protein in Akt signal pathway,and then increasing the expression of Bcl-2 and in-hibiting the expression of Bax.